Short report
Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor
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* Corresponding author: David A Jans david.jans@med.monash.edu.au
1 Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
2 Retroviral Genetics Division, Centre for Virus Research, Westmead Millennium Institute, Westmead Hospital, The University of Sydney, Darcy Road, Westmead, N.S.W 2145, Australia
3 HIV Protein Function and Interactions Group, Centre for Virus Research, Westmead Millennium Institute, Westmead Hospital, The University of Sydney, Darcy Road, Westmead, N.S.W 2145, Australia
4 University of Western Sydney, Penrith South DC, N.S.W 1797, Australia
Retrovirology 2008, 5:67 doi:10.1186/1742-4690-5-67
Published: 18 July 2008Additional files
Additional file 1:
LTNP derived Vpr proteins with reduced nuclear accumulation localize within the Golgi. Typical CLSM images of fixed COS-7 cells expressing the indicated GFP-fusion proteins. Cells were permeabilised and stained 14 hours post transfection for γ-adaptin and visualized with Alexa-Fluor-568. GFP-Vpr proteins specifically containing F72L show colocalisation with the Golgi apparatus as indicated by arrows.
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Additional file 2:
LTNP derived Vpr proteins with reduced nuclear accumulation localize within the Golgi. Typical CLSM images of fixed COS-7 cells expressing the indicated GFP-fusion proteins. Cells were permeabilised and stained 14 hours post transfection for Calnexin and visualized with Alexa-Fluor-568. GFP-Vpr was found to not localize within the ER.
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