Additional file 2.

LTNP derived Vpr proteins with reduced nuclear accumulation localize within the Golgi. Typical CLSM images of fixed COS-7 cells expressing the indicated GFP-fusion proteins. Cells were permeabilised and stained 14 hours post transfection for Calnexin and visualized with Alexa-Fluor-568. GFP-Vpr was found to not localize within the ER.

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Caly et al. Retrovirology 2008 5:67   doi:10.1186/1742-4690-5-67