Figure 2.

Summary tree showing the evolutionary relationships between all of the sequences used in this analysis, showing median node heights derived from 9000 posterior trees. Labels on the tips first show the sampling date in month and year and whether the generic (P for population) or L90M specific (M for mutant) PCR primers were used to generate the sequenced amplicons. The following number (1 through 6) represents the order of the bleeds analyzed. Patient numbers are given in the margin. Sequences that have the L90M mutation are indicated in darker bold fonts. Each patient forms a monophyletic cluster with 100% Bayesian posterior probability support (in 100% of posterior trees). Posterior support values for the monophyletic relationships of the sequences with the L90M mutation are given in Table 1. Patients with sequences showing evidence for multiple origins of L90M are shown in red, those with a single L90M origin in blue and those with an inconclusive number of L90M origins in black.