Figure 6.

Kinetics of ASV-NLS mediated import. GST-NLS fusion proteins were incubated in digitonin permeabilized HeLa cells for various times (labeled above each column) at 37°C prior to fixation with paraformaldehyde and staining with fluorescent antibody against GST. The fusion protein used in each row is labeled at the right and the properties described in the text. Fusion proteins that are imported with slower kinetics are grouped at the top (rows 1–4), and those with faster kinetics in the middle (rows 5 and 6). Control fusion proteins that are not imported into the nucleus are in rows 7 and 8.