Figure 5.

Effect of hNAP-1 on HIV-1 infection. A. Overexpression of hNAP-1 enhances LTR transcription upon HIV-1 infection. HeLa cells were transfected with an expression vector for HA-hNAP-1 or with a control vector, and then infected with VSG-luciferase HIV-1 vector. Luciferase activity was measured after 24 h post-infection. The mean ± s.d. of at least three different experiments is shown. The panel on the right side shows anti-HA western blottings to assess HA-hNAP-1 expression in a representative experiment. B. Silencing of hNAP-1 impairs LTR transcription upon HIV-1 infection. HeLa cells were treated with an siRNA directed against hNAP-1 or a control siRNA. Forty-eight hours after the beginning of siRNA treatment, cells were infected with the luciferase reported virus, and luciferase assays were performed on cell lysates 24 hours later. The mean ± s.d. of at least three different experiments is shown. The panel on the right side shows anti-hNAP-1 western blottings to assess the levels of endogenous hNAP-1 and tubulin expression in a representative experiment.