Replication-dependent core and linker histone transcripts are reduced in HTLV-1 infected T-cell lines. (A) Core and linker histone transcript levels were measured in uninfected T-cell lines (CEM, Jurkat and Molt4) and HTLV-1 infected T-cell lines (SLB-1, HuT102 and C81) by northern blotting. (B) Graphical representation of northern blot quantification. Histone transcript signals were normalized to EF1α transcript signals. Values for all T-cell lines are expressed relative to Jurkat cells. P-values associated with northern blot measurements of total histone transcripts for each cell line were: CEM – 2.3 × 10-3, Molt4 – 1.1 × 10-4, SLB-1 – 5.8 × 10-12, HuT102 – 2.9 × 10-8, C81 – 2.8 × 10-6 (C) Linker histone isoform transcripts are reduced in HTLV-1 infected T-cell lines. Somatic linker histone isoform transcript levels were measured in an uninfected T-cell line (Jurkat) and an HTLV-1 infected T-cell line (SLB-1), using reverse-transcription and real-time PCR. Linker histone transcript levels were normalized to EF1α transcript levels and values were expressed relative to transcript levels in Jurkat cells .
Bogenberger and Laybourn Retrovirology 2008 5:9 doi:10.1186/1742-4690-5-9