Figure 3.

Tax expression is sufficient for reduction of replication-dependent histone transcript levels. (A) Histone transcript levels were measured in pSG-Tax transfected Jurkat cells and empty vector transfected Jurkat cells using reverse-transcription and real-time PCR. P-values associated with the measurement of histone transcripts in pUC-19 vs pSG-Tax transfected Jurkat cells were: H2A – 3.7 × 10^-6, H2B – 3.4 × 10^-5, H3 – 5.6 × 10^-3, H4 – 3.5 × 10^-2, average H1 – 1.1 × 10^-2. (B) DNA content analysis reveals similar cell cycle distributions for pSG-Tax transfected and empty vector transfected Jurkat cells.