Figure 1.

Immunohistochemical detection of the HHV-8 LANA protein in placental histocultures. Specific reactivity was visualized with immunoperoxidase staining using anti-LANA-1 monoclonal antibodies with a DAB developer (brown colour) and haematoxylin counterstaining. (A) HHV-8-infected CRO-AP/3 cells showed a strongly positive nuclear immunostaining. (B) HHV-8-infected placental histocultures showed positive immunostaining in cytotrophoblasts (yellow arrow), syncytiotrophoblasts (blue arrow) and endothelial cells (white arrow). (C) Mock-infected placental histocultures. Original magnifications, X100.