Research
Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads
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* Corresponding author: Luc Willems willems.l@fsagx.ac.be
1 Université de Liège (ULg), Gembloux Agro-Bio Tech, Molecular and Cellular Biology, Gembloux, Belgium
2 National Veterinary Research Institute, Pulawy, Poland
3 Interdisciplinary Cluster for Applied Genoproteomics (GIGA), University of Liège (ULg), Liège, Belgium
Retrovirology 2009, 6:102 doi:10.1186/1742-4690-6-102
Published: 10 November 2009Additional files
Additional file 1:
Sheep hematological profiles.
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Additional file 2:
Kinetics of ROS production in B cells. PBMCs isolated from BLV-infected (n = 13) and non-infected (n = 7) sheep were seeded in 24-well plates at a density of 106 cells/ml and incubated for 30 min at 37°C with 10 μM of CM-H2DCFDA. After 3 h and 6 h of culture, B cells were stained using anti-IgM monoclonal (clone Pig45) and Alexa Fluor 647-conjugated donkey anti-mouse antibodies. The intracellular ROS levels were determined by flow cytometry and are presented as the mean fluorescence intensities (± standard deviation) of cellular chloromethyldichlorofluorescein (CM-DCF) within B cell populations.
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