Research
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity
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* Corresponding author: Tomasz Brudek tb@microbiology.au.dk
1 Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark
2 Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark
3 Department of Neurology, University of Aarhus, DK-8000 Aarhus C, Denmark
Retrovirology 2009, 6:104 doi:10.1186/1742-4690-6-104
Published: 16 November 2009Additional files
Additional file 1:
Supplementary figure 1. Flow cytometric analysis of surface expression of HERV-H and HERV-W Env epitopes on B-cells and monocytes from patients with active MS (AMS), stable MS patients (SMS), healthy individuals (HC), and neurological non-inflammatory controls (NC). The figure is a presentation of flow cytometric data analyses. From each group, two individuals representing high and low levels of HERV-H/-W Env epitope expression are shown. Blue peak - cells incubated with secondary goat anti-rabbit IgG, F(ab')2 FITC; green peak - cells incubated with appropriate pre-immune serum; red peak - cells incubated with anti-HERV-H/W Env TM/SU serum. The numbers in square brackets represent the fluorescence index calculated as the ratio of the mean fluorescence of the cells incubated with anti-Env Abs to the mean fluorescence of the cells incubated with the appropriate control (pre-immune serum).
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