Research
DNA double strand break repair enzymes function at multiple steps in retroviral infection
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* Corresponding author: Masao Matsuoka mmatsuok@virus.kyoto-u.ac.jp
1 Laboratory of Virus Control, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
2 Laboratory of Cell Regulation and Molecular Network, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan
3 Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
4 Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, 3-1-1, Asahi, Matsumoto, Nagano 390-8621, Japan
Retrovirology 2009, 6:114 doi:10.1186/1742-4690-6-114
Published: 15 December 2009Additional files
Additional file 1:
Figure S1. Transduction efficiency of an MLV-based vector into cells deficient in DSB repair enzymes. Description: The transduction efficiency of the MLV-based vector was drastically decreased in DNA-PKcs-deficient cells and decreased in Mre11-deficient cells, but not altered in ATM- and NBS1-deficient cells. (A) ATM-deficient cells and ATM-complemented cells were transduced with the MLV-based vector encoding a GFP reporter. 2 days postinfection, the percentage of GFP-positive cells was determined by flow cytometry. (B-D) The influence of DNA-PKcs (B), NBS1 (C) and Mre11 (D) on transduction efficiency of the MLV-based vector was investigated by the same method as in (A). Error bars represent +/- SD.
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Additional file 2:
Figure S2. Abnormal 3' junctions of the HIV-1 provirus in DNA-PKcs-deficient cells. Description: (A) Junctions between the 3' end of the provirus and the host DNA were analyzed in DNA-PKcs-deficient cells transduced with an HIV-based vector. Inserted abnormal sequences are in lower case. Abnormal nucleotides corresponding to the GT dinucleotides processed by integrase are presented in bold. (B) The number of junctions with insertions and/or deletions. The P values under the table are for comparison of the number of junctions with only insertions or both insertions and deletions to that of MRC5SV cells in Table 1.
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Additional file 3:
Table S1. Primers for the sequence analyses around retroviral integration sites.
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