Figure 4.

Gradient of A3G-induced hypermutation across proviral DNA, cellular viral RNA (cRNA), and virion RNA (vRNA) observed in the untranslated leader region (UTR) and the beginning of gag of HIV-YRHHY > A5. (A) Schematic representation of a sample of proviral DNA sequences of individual clones from Round 2. (B) Schematic representation of a sample of cRNA sequences of individual clones from Round 2. (C) Schematic representation of a sample of vRNA sequences of individual clones from Round 2. Samples were extracted as described in FIG. 3A–F legend. (D) Graphical representation of the G-to-A hypermutation frequency from Round 2 of infection. The frequency of G-to-A hypermutation in the proviral DNA, cRNA, and vRNA across each individual infection (YA, YB and YC) for Round 2 was determined and presented as described in FIG. 3G legend. (E) Graphical representation of the type of G-to-A mutations observed in each individual clone in the proviral DNA, the cRNA, and the vRNA. The analysis was carried out as described in FIG. 3H legend. For the proviral DNA, 24 sequences were analyzed, for the cRNA 116 sequences were analyzed, and for the vRNA 96 sequences were analyzed.