Figure 1.

Expression of hCycT1 in CD4 T-cells and macrophages from hCycT1-tg rats. (A) Schematic representation of the tg vector for hCycT1, pMΦE4A.CyclinT1. (For details, see "Methods" and [25]. E4/P4: murine CD4 enhancer/promoter. (B) hCycT1 transgene-specific PCR amplifying a diagnostic 1.7-kb fragment in tail DNA from hCycT1-tg rats. M: DNA marker. (C-E) Western blot analysis of hCycT1 expression in extracts from (C) thymocytes, (D) spleen-derived CD4 T-cells and CD8 T-cells, enriched by magnetic bead selection, or (E) spleen-derived macrophages, using an antibody specific for CycT1 of human origin. Cells from n-tg littermates, or human Sup-T1 T-cells and human MDMs served as negative and positive controls, respectively. (C, D) Blots were reprobed for ERK2 as loading reference. (D) * indicates the hCycT1-specific band. The lower band seen in all splenocyte samples was considered non-specific. # gives the ID numbers of individual tg rats.