Figure 10.

hCycT1 transgenesis boosts replication of HIV-1_R7/3 YU-2 Env GFP in T-cells from multi-tg rats. Activated T-cells derived from the spleens of four double- or four triple-tg rats were challenged with (A) single-round VSV-G HIV-1_NL4-3 GFP reporter virus and the MFI (GFP) of viable cells was quantified on day 3 p.i. (B) In parallel, T-cell cultures were challenged with HIV-1_R7/3 YU-2 Env GFP either in the absence (circles) or presence (triangles) of efavirenz (5 μM). Cultures were washed the following day and cultivated for 14 days. At the indicated time points p.i., the percentage of GFP-positive cells was determined by flow cytometry. Shown are the arithmetic mean ± SD of triplicates. In the group of hCD4/hCCR5/hCycT1-tg rats significantly higher replication levels were reached compared to the group of hCD4/hCCR5-tg rats (p = 0.02; linear model variance analysis).