Figure 9.

The spread of HIV-1_R7/3 YU-2 Env GFP in hCD4/hCCR5-tg rat T-cells is sensitive to a reverse transcriptase inhibitor and not seen for a second replication-competent R5 HIV-1 GFP reporter virus. Activated T-cells derived from a hCD4/hCCR5-tg rat and a human donor were challenged with either replication-competent HIV-1_R7/3 YU-2 Env GFP virus or replication-competent HIV-1_NL4-3 92TH014-2 Env GFP virus (each 500 ng p24 per 2 × 10⁶ cells). 18 h p.i. the infected wells were split and cultivation was continued in the presence or absence of efavirenz (EFV) (5 μM). At the indicated time points, the infection was analyzed for (A) the percentage of GFP-positive cells by flow cytometry and (B) the p24 concentration in culture supernatants by antigen ELISA. Data shown are arithmetic means ± SD of triplicates.