When is it time for reverse transcription to start and go?
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* Corresponding author: Jean-Luc Darlix jldarlix@ens-lyon.fr
Retrovirology 2009, 6:24 doi:10.1186/1742-4690-6-24
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BioMed Central: 4 citations
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Editorial
Jean-Luc Darlix: Renaissance scientist and retrovirologist par excellence Mark A Wainberg, Eric A Cohen Retrovirology 2011, 8:59 (18 July 2011) Jean-Luc Darlix was recently recognized with the first Retrovirology Lifetime Achievement Award on the occasion of his "retirement" symposium in Lyon.
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Research
James A Thomas, Teresa L Shatzer, Robert J Gorelick Retrovirology 2011, 8:46 (17 June 2011) In the present study we investigated whether blocking premature reverse transcription would relieve the infectivity defects, which we successfully performed by transfecting proviral plasmids into cells cultured in the presence of high levels of reverse transcriptase inhibitors. After subsequent removal of the inhibitors, the resulting viruses showed no significant difference in single-round infective titer compared to viruses where premature reverse transcription did occur; there was no rescue of the infectivity defects in the NC mutants upon reverse transcriptase inhibitor treatment. Surprisingly, time-course endogenous reverse transcription assays demonstrated that the kinetics for both the NC mutants were essentially identical to wild-type when premature reverse transcription was blocked. In contrast, after infection of CD4+ HeLa cells, it was observed that while the prevention of premature reverse transcription in the NC mutants resulted in lower quantities of initial reverse transcripts, the kinetics of reverse transcription were not restored to that of untreated wild-type HIV-1.
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Review
The macrophage in HIV-1 infection: From activation to deactivation? Georges Herbein, Audrey Varin Retrovirology 2010, 7:33 (9 April 2010) |
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Poster presentation
Bing Yu, Laurent Houzet, Ludovic Didierlaurent, Célia Chamontin, Zakia Morichaud, Jean Darlix, Marylène Mougel Retrovirology 2009, 6(Suppl 2):P14 (24 September 2009) |