Figure 8.

TLR4-mediated decrease in de novo virus production involves type-I IFNs. A) IM-MDDCs were either left untreated (mock) or stimulated for 6 hours with the listed TLR ligands. Cell-free supernatants were harvested and the levels of IFNα/β were quantified through the use of HEK-Blue™ IFNα/β cells. The data shown represent the mean ± standard deviations of quadruplicate samples from a single donor. B) IM-MDDCs were either left untreated (mock) or stimulated for 2 hours with the TLR4 ligand LPS. Cells were then washed twice, pulsed with NL4-3/Balenv and cultured in absence or presence of B18R (0.1 μg/ml). Supernatants were harvested at 72 hours post-infection and the viral content was evaluated by a p24 test. The data shown represent the mean ± standard deviations of quadruplicate samples from a single donor and are representative of 4 different donors.