Figure 7.

Impact on HIV-1 expression. Anti-HIV-1 suppressive activity was tested by transfecting each shRNA expression plasmid with the NL4-3 expression plasmid. Activities were measured as a reduction in, and expressed as percentage of, p24 production (measured as pg/ml) relative to p24 production from the unsuppressed control (shown in blue). The 96 shRNAs were measured in 3 sets (as indicated in purple) with 2 or more replicate experiments in each set (accessory gene shRNAs in one set, Gag plus Env shRNAs together, and Pol shRNAs separately).