Figure 10.

BiFC assays to evaluate interactions between RNA-binding competent A3G proteins and RNA-binding defective A3G proteins. (A) BiFC assays to evaluate interactions between mutant A3Gs. (B) Quantification of BiFC efficiency was determined by flow cytometry analysis as described in Fig. 2B. The percentage of YFP⁺ cells among the mRFP⁺ cells after cotransfection with A3G-NY and A3G-CY (15.3%) was set to 100%. The error bars represent the s.e.m. of two independent experiments. (C) Quantification of BiFC fluorescence mean intensity using flow cytometry analysis. The mean intensities of YFP⁺ cells were determined using CELLQUEST software. The error bars represent the s.e.m. of two independent experiments.