Figure 9.

BiFC assays to evaluate interactions between HIV-1 Gag, A3G, and RNA-binding defective mutants of Gag and A3G. (E) BiFC assays to evaluate interactions between HIV-1 Gag and RNA-binding defective A3G mutants H65R, F70A, and Y91A. (F) BiFC assays to evaluate interactions between HIV-1 Gag NC mutants and A3G. (G) Quantification of YFP fluorescence reconstitution with HIV-1 Gag and A3G BiFC constructs using flow cytometry analysis. The percentage of YFP⁺ cells in mRFP⁺ cells after co-transfection with Gag-NY and Gag-CY (3.5%) was set to 100%. The error bars represent the s.e.m. of two independent experiments.