Retrovirology

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Open Access Research

Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo

Stefanie Homann1, Nadine Tibroni1, Ingo Baumann2, Serkan Sertel2, Oliver T Keppler1 and Oliver T Fackler1*

Author Affiliations

1 Department of Virology, University of Heidelberg, Heidelberg, Germany

2 Department of Otolaryngology, Head and Neck Surgery, University of Heidelberg, Heidelberg, Germany

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Retrovirology 2009, 6:6 doi:10.1186/1742-4690-6-6

Published: 15 January 2009

Additional files

Additional File 1:

Supplementary figure one. Direct staining of CD4 was avoided due to the reduction of CD4 surface exposure in HIV-1 infected cells, but a control staining for mock infected cells reveals that virtually all CD3+/CD8- cells in this gate were positive for CD4.

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