Short report
Enhanced macrophage tropism of HIV in brain and lymphoid tissues is associated with sensitivity to the broadly neutralizing CD4 binding site antibody b12
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* Corresponding author: Dana Gabuzda dana_gabuzda@dfci.harvard.edu
1 Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston MA, USA
2 Department of Pathology, Harvard Medical School, Boston MA, USA
3 Department of Neurology, Harvard Medical School, Boston MA, USA
Retrovirology 2009, 6:69 doi:10.1186/1742-4690-6-69
Published: 20 July 2009Additional files
Additional file 1:
Supplementary Figure. HIV Env neutralization sensitivity to mAb b12 does not correlate neutralization sensitivity to sCD4. HIV luciferase reporter viruses were incubated with a range of concentrations of human mAb b12 or sCD4 1 h prior to infection of Cf2 cells transiently expressing CD4 and CCR5. Cells were harvested 48 h post infection and assayed for luciferase activity. Data are expressed as the concentrations at which luciferase expression was reduced by 50% compared to infection in the absence of mAb or sCD4 (IC50). (A) sCD4 IC50s were plotted as a function of b12 IC50s. R and p values, Spearman correlation. (B) sCD4 IC50s of HIV Envs with low to intermediate b12 sensitivity (< median; median = 3.374 μg/ml) were compared to Envs with intermediate to high b12 sensitivity (> median). p values, Mann-Whitney test.
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