Modification of a loop sequence between α-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vif and CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells
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* Corresponding author: Emi E Nakayama emien@biken.osaka-u.ac.jp
Retrovirology 2009, 6:70 doi:10.1186/1742-4690-6-70
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Ayumu Kuroishi, Katarzyna Bozek, Tatsuo Shioda, Emi E Nakayama Retrovirology 2010, 7:58 (7 July 2010) By long-term cultivation of human CEMss cells infected with NL-4/5S6/7SvifS, the impaired replicative capability of the virus in human cells was rescued. Sequence analysis of the CA region of the adapted virus revealed a G-to-E substitution at the 116th position of the CA (G116E). Introduction of this substitution into the molecular DNA clone of NL-4/5S6/7SvifS indeed improved the virus' replicative capability in human cells.
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