Table 1

DNA oligonucleotides used in the present study

Oligo DNA

Sequence and position on Tat mRNA

LeaderR sense EcoRI

5' GAATTCGGTCTCTCTGGTTAGACCAGATC 3'

(exon1 sense for Tat1 et Tat2: 1→)

Leader rev1

5' TATTCTGCTATGTCGACACCCAATTCAGTCGCCGCCCCTCG 3'

(exon1 reverse for Tat1:← 290)

Leader rev2

5' GGATCTCTGCTGTCCCTGCAGTCGCCGCCCCTCG 3'

(exon1 reverse for Tat2:← 290)

Ex2Tat sense

5' AATTGGGTGTCGACATAGCAGAATAGGCG 3'

(exon2 sense for Tat1:290→ and for Tat2:343→)

Ex2Tat rev

5'GGGATTGGGAGGTGGGTTGCTTTGATAGAGAAGCTTGATGAGTCTGACTG3' (exon2: reverse for Tat1:← 558 and for Tat2:← 611)

Ex'Tat sense

5'CAGGGACAGCAGAGATCCAGTTTGGAAAGGACCAGCAAAGCTCCTCTGGAAAG 3' (exon':sense for Tat2:290→)

Ex'Tat rev

5' CTGCTATGTCGACACCCAATTCTTTCCAGAGGAGCTTTGCTG 3'

(exon':reverse for Tat2:← 343)

Ex3Tat sense

5' ACCCACCTCCCAATCCCGAG 3'

(exon3: sense for Tat1:558→ and for Tat2: 611→)

Tatrev NotI

5' TAATAATGCGGCCGCAGTACAGGCAAAAAGCAGCTGCTTATATGC 3'

(exon3: reverse for Tat1:← 1718 and for Tat2:← 1770)

PBSEcoRI sense

5' TATATTAGAATTCGTGTGCCCGTCTGTTGTGTGACT 3'

(exon1: sense for Tat1 and for Tat2:104 →)

SDEcoRI sense

5' ATATAAGAATTCCGAGGGGCGGCGACTG 3'

(exon1: sense for Tat1 and for Tat2:274→)

AUGEcoRI sense

5' TATAATAGAATTCATGGAGCCAGTAGATCCTAGACTAGAG 3'

(exon2: sense for Tat1:343 → and for Tat2:396 →)

TatNcoI sense

5' TAATATACCATGGGGTCTCTCTGGTTAGACCAGATC 3'

(exon1: sense for Tat1 and for Tat2:1→)

TatSmaI rev

5' TATATACCCGGGAGTACAGGCAAAAAGCAGCTGCTTATATGC 3'

(exon3: reverse for Tat1:← 1718 and for Tat2:← 1770)

TatXbaI sense

5' ATATATTCTAGAGGTCTCTCTGGTTAGACCAGATC 3'

(exon 1:for Tat1 et Tat2:1→)

TatXbaI rev

5' TAATAATTCTAGAAGTACAGGCAAAAAGCAGCTGCTTATATGC 3'

(exon3 reverse for Tat1:← 1718 and for Tat2:← 1770)

(111)NcoI rev

5' TATATACCATGGGGCACACACTACTTTGAGCACTCAAGG 3'

(exon1:reverse:← 111)

UTRTatNcoI rev

5' TATATTACCATGGTTCTTGCTCTCCTCTGTCGAGTAACG 3'

(exon2: reverse for Tat1:← 342 and for Tat2:← 395)

(1-336)NcoI rev

5' ATATATACCATGGCTCTCTCCTTCTAGCCTCCGC 3'

(reverse: 5' to AUG of the RNAg)

UTR2TatNcoI sense

5' TAATATACCATGGGTGTGCCCGTCTGTTGTGTGACT 3'

(exon1: sense for Tat1 and for Tat2:104 →)

LTRPvuII sense

5' TAATATACAGCTGTGGAAGGGCTAATTTGGTCCC 3' (sense for U3)

LTRPvuII rev

5' TATATTACAGCTGAGTACAGGCAAAAAGCAGCTGC 3' (reverse for U3)

Charnay et al. Retrovirology 2009 6:74   doi:10.1186/1742-4690-6-74