Commentary
siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication
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* Corresponding author: Dong-Yan Jin dyjin@hkucc.hku.hk
Department of Biochemistry, the University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, PR China
Retrovirology 2009, 6:78 doi:10.1186/1742-4690-6-78
Published: 27 August 2009Abstract
A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication. This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.