Table 5 |
||
|
Primers used to generate XPR1 mutants. |
||
|
Mutant |
Primer sequence (5'→3') |
GenBank number |
|
|
||
|
KPYK, ESTV |
1F: AAATCCAGATTTTGGCTGCTCAA (1315-1337) 1R: GCAGGCACTGGATGAAGCGAA (1583-1603) 2F: TTCGCTTCATCCAGTGCCTGC (1583-1603) 2R: AAGAGACCCCAGTCCATCTTGA (1799-1820) |
|
|
|
||
|
S505P |
F: CACGAAGAACAAAATCACCCTGACACCGTGGTGTTCT (1705-1741) R: AGAACACCACGGTGTCAGGGTGATTTTGTTCTTCGTG (1705-1741) |
|
|
|
||
|
T507Y |
F: AGAACAAAATCACTCTGACTACGTGGTGTTCTTTTA CCTGTGG (1710-1752) R: CCACAGGTAAAAGAACACCACGTAGTCAGAGTGATT TTGTTCT (1710-1752) |
|
|
|
||
|
P505S |
F: CACAAAGAACAAAATCACTCTGACTACAAGGTGTTC (1495-1530) R: GAACACCTTGTAGTCAGAGTGATTTTGTTCTTTGTG (1495-1530) |
|
|
|
||
|
Y507T |
F: AGAACAAAATCACCCTGACACCAAGGTGTTCTTTTA CCTGTGG (1500-1542) R: CCACAGGTAAAAGAACACCTTGGTGTCAGGGTGATT TTGTTCT (1500-1542) |
|
|
|
||
|
TV |
F: AGAACAAAATCACCCTGACACCGTGGTGTTCTTTTAC CTGTGG (1500-1542) R: CCACAGGTAAAAGAACACCACGGTGTCAGGGTGATT TTGTTCT (1500-1542) |
|
|
|
||
|
Underlined letters represent introduced base substitutions. Numbers in parentheses indicate the position of each primer in the indicated GenBank sequence. |
||
|
Yan et al. Retrovirology 2009 6:87 doi:10.1186/1742-4690-6-87 |
||
