This article is part of the supplement: Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts

Invited speaker presentation

Post-entry events of efficient R5 vs. inefficient X4 HIV-1 replication in primary CD4⁺T lymphocytes, a transcriptome analysis

Samanta A Mariani¹, Andrea Crotti¹, Elisa Vicenzi¹, Huang Dawei², Richard Lempicky², Claudia Cicala³, James Arthos³ and Guido Poli^1*

• * Corresponding author: Guido Poli

Author Affiliations

¹ Department of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano 20132, Italy

² Science Applications International Corporation, Frederick, MD 21702, USA

³ National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA

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Retrovirology 2009, 6(Suppl 2):I19 doi:10.1186/1742-4690-6-S2-I19

The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/6/S2/I19

Published:

24 September 2009

© 2009 Mariani et al; licensee BioMed Central Ltd.

Invited speaker presentation

HIV-1 infects CD4+ cells via interaction with CD4 and either CCR5 or CXCR4. However, only CCR5-using (R5) viruses are efficiently transmitted and sustain the viral pandemics, while CXCR4-using (X4) viruses emerge later in coincidence with the immunodeficiency state and progression to AIDS in about 50% of individuals infected with subtype B HIV-1, but not with other subtypes. Unraveling cellular and molecular correlates of this asymmetric co-receptor use would be relevant to understand HIV pathogenesis as well as for the development of preventive strategies aimed at blocking R5 HIV-1 spreading. We have previously reported that cord blood derived CD4⁺T cells (CB4 cells) maintained in a sub-optimally activated state in IL-2 enriched medium for 7-14 days before infection are permissive for R5 and restricted for X4 HIV-1 replication. Of interest, this restriction did not occur at the level of viral entry, but it was rather correlated to a superior capacity of R5 HIV-1 to spread after infection [1]. In the present study, we examined the transcriptomic profile at different time points (8, 24, 48, 72 h) of CB4 cells established from 6 independent donor/infection pairs after infection with isogenic NL4-3 (X4) and NL-AD8 (R5) viruses normalized for MOI. Gene expression was measured using Human Genome U95A chips and analyzed with the DAVID knowledge base software. Approximately 900 and 1,100 genes were selectively mobilized by R5 and X4 HIV-1 infection, respectively, vs. mock-stimulated uninfected control cells. An additional 420 genes were modulated by both viruses vs. controls. R5 HIV-1 induced a rapid mobilization of genes linked to cell proliferation and signal transduction, whereas the X4 virus predominantly modulated the expression of genes associated with cell death and the immune response. Both viruses upregulated the expression of CXCL12/SDF-1α, but only X4 downregulated CXCR4 mRNA; CCR5 mRNA was unaffected by either infection at all time points. Other genes previously linked to control of HIV replication that were modulated by R5 and X4 HIV-1 include APOBEC-3G, IFN-γ, CCL5/RANTES, CCL7/MCP-3 and CCL14/HCC1. We are currently analyzing additional genes discordantly co-modulated by R5 and X4 viruses in the search of host genes associated with the permissive vs. restricted HIV replicative profile in this model system. Thus, both R5 and X4 HIV-1 profoundly affect the transcriptional activity of primary CD4⁺T lymphocytes even in the absence of overt replication (as observed in X4 infection).

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