Examples of BLT positive serum neutralisation activity. A, Infectivity of XMRV (measured as counts per second of chemiluminescence produced from β-galactosidase activity) after incubation with patient serum or hybridoma cell supernatant. Infectivity is plotted against the reciprocal dilution of the BLT serum (black circles, top panel, sample Q488, bottom panel, sample Q610; triangles, negative control, monoclonal 603; squares, positive control, monoclonal 83A25'). The dashed line indicates viral infectivity in the absence of sera. B, Infectivity data for viruses with four different envelopes (circles, XMRV; squares, MLV(X); triangles, Friend-MLV; crosses, VSV-G) after incubation with patient serum. Data were normalised by setting the infectivity for each virus in the absence of patient serum at 100%, and plotted against the reciprocal of serum dilution for two positive sera, top panel sample Q488 and bottom panel sample Q610.
Groom et al. Retrovirology 2010 7:10 doi:10.1186/1742-4690-7-10