Table 1 |
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|
Replication capacity of HIV-1 with mutations in the p51↓RNH cleavage site ± T477A |
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|
RT p51↓RNH mutation |
Virus titer (% wild-type control)a |
||
|
- T477A |
+ T477A |
||
|
|
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|
WT |
100 |
100 ± 30 |
(N.S.)b |
|
F440V |
< 1 ± 1 |
10 ± 3 |
(p < 0.01) |
|
T439S/V442G |
---c |
--- |
|
|
Y441I/V442K |
--- |
--- |
|
|
F440A |
<1 ± 1 |
10 ± 5 |
(p < 0.01) |
|
F440A/Y441A |
<1 ± 1 |
1 ± 1 |
(N.S.) |
|
F440W/Y441W |
<1 ± 1 |
32 ± 14 |
(p < 0.01) |
|
E438N |
<1 ± 1 |
5 ± 4 |
(p < 0.5) |
|
|
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|
a Infectious virus titer (TCID50/ml) was determined in MT-2 cells and was normalized by dividing by the amount of viral p24 (ng/ml), as described in Materials and Methods. Data are presented as mean % wild-type virus titer ± standard deviation from four individual experiments. b P values were calculated using a one-tailed Student's t-test assuming equal variance. N.S., not significant. c No virus titer noted |
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|
Abram et al. Retrovirology 2010 7:6 doi:10.1186/1742-4690-7-6 |
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