Research
Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RNA
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* Corresponding author: Mario PS Chin mchin@adarc.org
Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York, USA
Retrovirology 2010, 7:73 doi:10.1186/1742-4690-7-73
Published: 8 September 2010Additional files
Additional file 1:
Supplemental Figure S1. Replication of NL4-3 and NLΔSL1 in PM-1 cells as determined by p24 ELISA. PM-1 cells were infected with p24-normalized NL4-3 or NLΔSL1. Culture supernatants from the infected PM-1 were collected at different times, and p24 levels were measured by ELISA.
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Additional file 2:
Supplemental Figure S2. Predicted secondary structures of NL4-3 and SL1 deletion mutants. Genomic RNA of (A) NL4-3, (B) NL-913 and (C) NL-1907 (nt 456 to 2080) and (D) NLΔSL1, (E) NLΔSL1-913 and (F) NLΔSL1-1907 (nt 456 to 2037) were subjected to Mfold analysis. The SL1 and SL2 and the positions of the MA (913) and SP1 (1907) substitutions are labeled.
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