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A whole genome screen for HIV restriction factors

Li Liu1, Nidia MM Oliveira1†, Kelly M Cheney1†, Corinna Pade1, Hanna Dreja1, Ann-Marie H Bergin2, Viola Borgdorff2, David H Beach2, Cleo L Bishop2, Matthias T Dittmar1 and Áine McKnight1*

1 Centre for Immunology and Infectious Disease, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK

2 Centre for Cutaneous Research, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK

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Retrovirology 2011, 8:94 doi:10.1186/1742-4690-8-94

Published: 14 November 2011

Additional files

Additional file 1:

Efficiency of siRNA knockdown on HIV infection. siRNA knockdown of the HIV receptor CD4 and nuclear importin TNPO3 inhibits infection of HIV-18.2N by 95.8% and 93% respectively compared with CB control siRNA. Green, virus; red, cells.

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Additonal file 2:

Results of primary screen with Z-scores. Positive results from primary screen are listed. Z-scores are given for all hits greater than 3SD from CB control siRNA.

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Additional file 3:

siRNA knockdown of 114 genes rescues HIV infection. Infection of HIV89.6R pseudotyped virus was rescued in HeLa-CD4 cells following siRNA knockdown of 114 genes. The y-axis denotes both the number of infectious viruses rescued (focus forming units/ml, FFU/ml; left) and fold rescue compared with the CB control (right). FFU/ml results are mean ± SD.

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Additional file 4:

Pathway analysis of most potent screen hits. Ingenuity Pathway Analysis (IPA http://www.ingenuity.com webcite) was performed on the validated screen hits showing the functions, if known, associated with the most potent.

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Additional file 5:

Network, functional and pathway analysis. A list of 114 confirmed factors including heat map and GO terms.

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