Validation of screen positive results. Distribution of rescued HIV89.6R infection determined by GFP+ foci/well (left Y axis) with Z-scores (right Y axis) for the siRNA library (open circles) compared with CB control siRNA (closed circles). AP2M1, DNM2, SETDB1 and genes associated with the PAF1c CTR9, PAF1 and RTF1 are highlighted. GFP+ foci more than 3 SD (equivalent to 7.43 foci) from the average of control infections are shown.
Liu et al. Retrovirology 2011 8:94 doi:10.1186/1742-4690-8-94