Analysis of selected genes. 3A AP2M1 and DNM2 siRNA knockdown rescues infection of HeLa-CD4 cells by pseudotyped HIV89.6R and wild type HIV-189.6wt and HIV-1NL4.3wt. The y-axis denotes both the number of infectious viruses rescued (FFU/ml; left) and fold rescue compared with the CB control (right). FFU/ml results are mean ± SD. 3B SETBD1 siRNA knockdown rescues infection of HeLa-CD4 cells by replication competent HIV-189.6wt and HIV-1NL4.3wt (MOI 0.7). FFU/ml results are mean ± SD. 3C Knockdown of SETDB1 results in enhanced HIV-1 RT products - total (0-16 hr pi) and integrated proviral DNA (Alu-gag, fold change, 48 hr pi). Control wells containing inhibitors were negative for HIV-1 DNA. HIV-1 DNA copies are normalised to genomic GAPDH and presented per million cells. Results are mean ± SD of a representative experiment performed in duplicate.
Liu et al. Retrovirology 2011 8:94 doi:10.1186/1742-4690-8-94