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This article is part of the supplement: 15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access Meeting abstract

The utilization of humanized mouse models for the study of inhibitors in HTLV-1 infection

Rachel Van Duyne12, Irene Guendel1, Kylene Kehn-Hall1, Mohammed Saifuddin3 and Fatah Kashanchi12*

Author Affiliations

1 George Mason University, Department of Molecular and Microbiology, National Center for Biodefense and Infectious Diseases, Manassas, VA, 20110, USA

2 The George Washington University Medical Center, Department of Microbiology, Immunology, and Tropical Medicine, Washington, DC, 20037, USA

3 CONRAD, Eastern Virginia Medical School, Arlington, VA, 22209, USA

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Retrovirology 2011, 8(Suppl 1):A28  doi:10.1186/1742-4690-8-S1-A28


The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/8/S1/A28


Published:6 June 2011

© 2011 Van Duyne et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Meeting abstract

The development of novel techniques and systems to study human infectious diseases in both an in vitro and in vivo settings is always in high demand. Ideally, small animal models are the most efficient method of studying human afflictions. This is especially evident in the study of the human retroviruses, HIV-1 and HTLV-1, in that current simian animal models, though robust, are often expensive and difficult to maintain. Recently significant advances have been made to use human stem cells in immunocomprimised animals and follow the course of infection. HTLV-1-infected humanized non-obese diabetic severe combined immunodeficiency (HU-NOD/SCID) mice have been shown by inoculation of NOD/SCID mice with CD34(+) hematopoietic progenitor and stem cells (CD34(+) HP/HSCs) infected ex vivo with HTLV-1 [1]. These mice exclusively develop CD4 (+) T-cell lymphomas with characteristics similar to ATL and elevated proliferation of infected human stem cells in the bone marrow were observed in mice developing malignancies. We will discuss the results of a panel of inhibitors against NFkB, cyclin/cdk complexes and Jack/Stat pathway that effectively inhibit HTLV-1 and Tax expression in vivo.

References

  1. Banerjee P, Tripp A, Lairmore MD, Crawford L, Sieburg M, Ramos JC, Harrington W Jr, Beilke MA, Feuer G: Adult T-cell leukemia/lymphoma development in HTLV-1-infected humanized SCID mice.

    Blood 2010, 115(13):2640-2648. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL