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This article is part of the supplement: Frontiers of Retrovirology 2011

Open Access Oral presentation

Role of cellular factors in the replication of human retroviruses: recent insights

Kuan-Teh Jeang

  • Correspondence: Kuan-Teh Jeang

Author Affiliations

National Institutes of Health, Bethesda, MD 20892, USA

Retrovirology 2011, 8(Suppl 2):O21 doi:10.1186/1742-4690-8-S2-O21


The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/8/S2/O21


Published:3 October 2011

© 2011 Jeang; licensee BioMed Central Ltd.

Oral presentation

We and others have described the use of genome-wide screenings for cellular factors that contribute to HIV-1 replication [1,2]. One aspect of the HIV-1 life cycle important to viral replication is the post-transcriptional regulation of HIV-1 gene expression. In HIV-1 biology, a key step in gene expression is the post-transcriptional export of intron-containing viral RNAs which bypass the normal mechanism(s) that retain cellular intron-containing RNAs in the nucleus. Specific signals on the viral RNAs, such as instability sequences (INS) and Rev responsive element (RRE), are binding sites for viral and cellular factors that serve to regulate RNA-export. The HIV-1 encoded viral Rev protein binds to the RRE found on unspliced and incompletely spliced viral RNAs. Binding by Rev directs the export of these RNAs from the nucleus to the cytoplasm. Previously, Rev co-factors have been found to include cellular factors such as CRM1, DDX3, PIMT and Matrin3 [3,4]. I will discuss these and other cellular factors that influence HIV-1 replication.

References

  1. Yeung ML, Houzet L, Yedavalli VS, Jeang KT: A Genome-wide Short Hairpin RNA Screening of Jurkat T-cells for Human Proteins Contributing to Productive HIV-1 Replication.

    J Biol Chem 2009, 284:19463-73. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Kok KH, Lei T, Jin DY: siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication.

    Retrovirology 2009, 6:78. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  3. Yedavalli VSRK, Jeang KT: Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression.

    Retrovirology 2011, 8:61. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  4. Kula A, Guerra J, Knezevich A, Kleva D, Myers MP, Marcello A: Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function.

    Retrovirology 2011, 8:60. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL