Figure 1.

HIV-1_LAI with an SOS-linked Env is replication-defective. A. Schematic representation of the A501C and T605C single and double (SOS) mutants used in this study. Free cysteines with a sulfhydryl group are indicated by SH and an intermolecular disulfide bond between gp120 and gp41 is indicated by SS. B. 375 × 10³ MT-2 T cells were infected with 1.5 ng CA-p24 of C33A-produced virus and virus spread was monitored for 7 days by CA-p24 ELISA.