Figure 7.

Sensitivity of recombinant viruses carrying primary envelope sequences to entry inhibitors. Clonal viruses with R5-tropism exhibiting a spectrum of infectivities towards U373-R5 cells (panel A) and/or MT4-R5 cells (panel B) were selected from among those obtained from patients 1–4. For each of these 20 viruses, the IC50s were determined on U373-R5 cells for soluble CD4 (C), enfuvirtide (E), TAK-799 (G), and neutralizing monoclonal antibodies 2F5 (D), 2G12 (F), and 48d (H). Each symbol represents the mean of three independent determinations. For all inhibitors except TAK-799, significant patient-specific differences in IC50 were observed using the Kruskal-Wallis test. Brackets indicate significant pairwise differences in post-test comparisons performed using Dunn's multiple comparison test (* p < 0.05; ** p < 0.01). For patient 2, clonal viruses were obtained from plasma samples obtained at both 26 months (solid symbols) and 34 months (open symbols) after initial diagnosis.