Table 2 |
|||||
|
Amino acid heterogeneity of V3 region of HIV-1 proviral quasispecies harboured by monocytes (CD36) and T lymphocytes (CD26) and of plasma virions replicating in the two cell types after HAART interruption. |
|||||
|
Patient |
Sample type |
N. of unique variants* |
Diversity** |
Complexity*** |
|
|
|
|||||
|
Pt.1 |
Provirus |
CD36 |
161 |
0.0795 ± 0.0005 |
0.3798 |
|
|
|||||
|
CD26 |
226 |
0.0197 ± 0.0001 |
0.2119 |
||
|
|
|||||
|
Pt.2 |
Provirus |
CD36 |
231 |
0.1446 ± 0.0005 |
0.3620 |
|
|
|||||
|
CD26 |
59 |
0.0031 ± 0.0001 |
0.0919 |
||
|
|
|||||
|
Pt.3 |
Provirus |
CD36 |
46 |
0.0508 ± 0.0003 |
0.3042 |
|
|
|||||
|
CD26 |
3 |
0.0084 ± 0.0002 |
0.0738 |
||
|
|
|||||
|
Virus |
CD36 |
26 |
0.0428 ± 0.0010 |
0.2979 |
|
|
CD26 |
158 |
0.0298 ± 0.0003 |
0.3293 |
||
|
|
|||||
|
Pt.4 |
Provirus |
CD36 |
76 |
0.0226 ± 0.0007 |
0.1127 |
|
|
|||||
|
CD26 |
14 |
0.0457 ± 0.0003 |
0.1755 |
||
|
|
|||||
|
Virus |
CD36 |
15 |
0.0113 ± 0.0002 |
0.1138 |
|
|
|
|||||
|
CD26 |
41 |
0.0208 ± 0.0002 |
0.1222 |
||
|
|
|||||
|
Pt.5 |
Provirus |
CD36 |
157 |
0.0585 ± 0.0003 |
0.2756 |
|
|
|||||
|
CD26 |
120 |
0.0411 ± 0.0004 |
0.4233 |
||
|
|
|||||
|
Virus |
CD36 |
305 |
0.0728 ± 0.0005 |
0.3664 |
|
|
|
|||||
|
CD26 |
38 |
0.0042 ± 0.0001 |
0.0870 |
||
|
|
|||||
|
Sequences obtained by ultra-deep pyrosequencing were filtered through the correction algorithm and amino acid sequences obtained were analyzed to establish diversity and complexity, as described in the Materials and Methods section. * Number of unique sequences obtained after dereplication ** Diversity: p distance ± SEM *** Complexity: normalized Shannon entropy |
|||||
|
Rozera et al. Retrovirology 2009 6:15 doi:10.1186/1742-4690-6-15 |
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