Research
Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies
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* Corresponding author: Xiaoxing Qiu xiaoxing.qiu@abbott.com
1 Infectious Diseases R&D, Abbott Diagnostics, 100 Abbott Park Rd, Abbott Park, IL, 60064, USA
2 Department of Pathology, Emory University School of Medicine/Yerkes National Primate Research Center, 954 Gatewood Dr, Atlanta, GA, 30329, USA
3 Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Av, Cleveland, OH, 44195, USA
4 Glickman Urological and Kidney Institute and LRI, Cleveland Clinic Foundation, 9500 Euclid Av, Cleveland, OH, 44195, USA
Retrovirology 2010, 7:68 doi:10.1186/1742-4690-7-68
Published: 17 August 2010Additional files
Additional file 1:
WB analysis of CMIA reactive blood donor samples. A1, p15E CMIA reactive blood donor, A2, gp70 CMIA reactive blood donors and A3 p30 CMIA reactive blood donors were analyzed by WB with native XMRV viral proteins (4 μg/strip). A4, gp70 CMIA reactive blood donors were analyzed by WB with mammalian expressed recombinant gp70 WB. Primate bleed (RIl-10) and anti-MuLV pAb were used as positive controls.
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Additional file 2:
Schematic diagrams of XMRV CMIA assay formats. B1, Indirect (anti-human) 2-step assay format. B2, Direct (double antigen sandwich) 2-step assay format. B3, Direct (double antigen sandwich) 1-step assay format. rAgs = recombinant antigens; hv = chemiluminescent signal.
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