Email updates

Keep up to date with the latest news and content from Retrovirology and BioMed Central.

This article is part of the supplement: 15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access Meeting abstract

Direct detection of diverse metabolic changes in virally transformed and Tax-expressing cells by mass spectrometry

Prabhakar Sripadi1, Bindesh Shrestha1, Rebecca Easley2, Lawrence Carpio2, Kylene Kehn-Hall2, Sebastien Chevalier3, Renaud Mahieux4, Akos Vertes1 and Fatah Kashanchi25*

Author Affiliations

1 The George Washington University, Department of Chemistry, W. M. Keck Institute of Proteomics Technology and Applications, Washington, DC, 20037, USA

2 George Mason University, Department of Molecular and Microbiology, National Center for Biodefense and Infectious Diseases, Manassas, VA, 20110, USA

3 National Institutes of Health/National Cancer Institute, Laboratory of Cellular Oncology, Bethesda, Maryland, 20892, USA

4 Ecole Normale Supérieure de Lyon, Equipe Oncogenèse Rétrovirale, U758 INSERM, Lyon, France

5 The George Washington University Medical Center, Department of Microbiology, Immunology, and Tropical Medicine, Washington, DC, 20037, USA

For all author emails, please log on.

Retrovirology 2011, 8(Suppl 1):A179  doi:10.1186/1742-4690-8-S1-A179

The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/8/S1/A179


Published:6 June 2011

© 2011 Sripadi et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Meeting abstract

HTLV-1- induced transformation causes extensive changes at the gene, protein and metabolite levels. These changes are usually followed by gene-expression profiling and proteomic analysis. Exploring the metabolic consequences of viral transformation adds to the picture because the viruses rely on the metabolic network of their cellular hosts for survival and replication. Metabolites are small molecules of diverse physico-chemical properties with greatly different abundance levels that make their analysis challenging. Typically optical (e.g., Fourier transform infrared spectrometry), nuclear magnetic resonance (NMR) and mass spectrometric techniques in combination with separation techniques, such as gas chromatography, high performance liquid chromatography (HPLC) and capillary electrophoresis, have been used for metabolomic studies. Here we utilized a new and novel method called laser ablation electrospray ionization (LAESI) to detect metabolites without any processing of samples. When using the LAESI technique to identify metabolic changes in HTLV1 and Tax1 transformed T lymphocytes and in HTLV3 and Tax3 cells, we found virus type specific (HTLV1 vs. HTLV3), expression specific (Tax1 vs. Tax3) and cell type specific (T lymphocytes vs. kidney epithelial cells) changes in the metabolite profiles. We have identified a number of metabolites that are known in the literature to be deregulated in the viral transformation process (e. g., arginine, cAMP, glutathione) as well as multiple novel metabolites that may have implications in HTLV1-induced transformation (e. g., putrescine, N-acetyl aspartic acid, methoxytyramine). These new findings point to metabolic pathways that have a heretofore unexplored role in the viral transformation of host cells.