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This article is part of the supplement: 15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access Meeting abstract

In vitro assembly of xenotropic murine leukemia virus-related virus CA-NC protein

Romana Hadravová1, Jitka Štokrová1, Michal Doležal1, Iva Pichová1, Tomáš Ruml2 and Michaela Rumlová1*

Author Affiliations

1 Department of Biochemistry, Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences, Prague, Czech Republic

2 Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic

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Retrovirology 2011, 8(Suppl 1):A236  doi:10.1186/1742-4690-8-S1-A236

The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/8/S1/A236


Published:6 June 2011

© 2011 Hadravová et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Meeting abstract

Using in vitro expression/assembly system we studied the formation of virus-like particles Xenotropic Murine Leukemia Virus-related virus (XMRV). XMRV is novel human gammaretrovirus discovered in association with human prostate tumors. The genome organization is typical for gammaretroviruses consisting of two overlapping ORFs coding for Gag-Pro-Pol and Env polyproteins. The predicted Gag polyprotein consists of 536 amino acids and is separated from the Pro-Pol sequence by UAG stop codon.

Based on the amino acids similarities between MLV and XMRV Gag polyproteins, we designed primers bordering CA-NC region of Gag. Resulting PCR fragment was cloned into pET22b vector for expression of CA-NC in E. coli. We found that purified XMRV full-length CANC, starting with the conserved proline residue at the N-terminus of CA, was not able to assemble into particles. However, a modification of the N-terminus of CANC (modCANC) enabled formation of spherical particles. Moreover, the negative staining of the in vitro assembled particles of XMRV modCANC revealed different organization of protein layers in comparison to CA-NC of M-PMV.