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This article is part of the supplement: Frontiers of Retrovirology 2011

Open Access Poster presentation

Regulatory role of upstream AUG codons within the SIVmac239 genome

Gisela J van der Velden*, Atze T Das and Ben Berkhout

  • * Corresponding author: Gisela J van der Velden

Author Affiliations

Laboratory of Experimental Virology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, The Netherlands

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Retrovirology 2011, 8(Suppl 2):P73  doi:10.1186/1742-4690-8-S2-P73


The electronic version of this article is the complete one and can be found online at: http://www.retrovirology.com/content/8/S2/P73


Published:3 October 2011

© 2011 van der Velden et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Simian immunodeficiency virus from Rhesus macaques (SIVmac) is a primate lentivirus that exhibits extensive similarities with human immunodeficiency viruses (HIVs) in morphology, genome organization and biological properties. Like HIV, SIV is dependent on the host cellular machinery for transcription, translation and protein production. Alternative RNA splicing generates many mRNAs that allow the expression of all viral proteins. Consistent with the idea that translation occurs predominantly via a cap-dependent scanning mechanism, one usually does not find AUGs upstream of the open reading frames (ORFs) in HIV and SIV.

In SIVmac239, the envelope (Env) glycoprotein is translated from a 4 kb mRNA that also contains the upstream ORF (uORF) for Rev. It is currently accepted that the level of Env expression is dependent on leaky scanning due to suboptimal translation initiation at the upstream Rev AUG. Interestingly, another potential start codon is present immediately upstream of the Rev-AUG. We also identified an alternative Rev-Env mRNA that has in fact four upstream AUGs, raising questions about the regulation of Rev and Env translation. We constructed subgenomic Rev-Env reporter mRNAs to analyze how the different upstream AUGs affect protein expression. Our results indicate that the virus uses these unique upstream AUG codons to modulate the level of translation of the Rev and Env proteins.